This site offers educational materials (in English and Spanish*) for the poultry industry to support implementation of biosecurity recommendations identified in the Checklist for Self‐Assessment of Implementing Poultry Biosecurity. (Updated July 2018!) These materials reflect the items included in Standard E-Biosecurity Principles within the National Poultry Improvement Plan (NPIP) Official Program Standards. The Standard E-Biosecurity Principles apply to flocks that participate in the NPIP, which include, but are not limited to, broilers, turkeys, egg layers, commercial waterfowl, and upland gamebirds, that meet specified flock size requirements. The biosecurity principles in this checklist can also apply to flocks of other sizes and management types.

pdf_icon Checklist for Self‐Assessment of Implementing Poultry Biosecurity
pdf_icon Checklist for Self‐Assessment of Implementing Poultry Biosecurity – Spanish*

The materials are arranged by checklist item. The materials can be downloaded, printed and used as is. However, poultry Biosecurity Coordinators are encouraged to modify the resources to best meet the situation at their operations. Note, if the text appears shadowed or hard to read when previewing the file, download the file and open it locally. That should resolve the issue.

These HPAI Biosecurity Training Materials were produced by the Center for Food Security and Public Health, Iowa State University, College of Veterinary Medicine. The USDA, APHIS, Veterinary Services, Surveillance, Preparedness and Response Services, National Preparedness and Incident Coordination provided funding through a cooperative agreement to the Center for Food Security and Public Health to develop these materials. These materials may not necessarily express APHIS’ views.

We welcome your suggestions for improvement.

*Spanish materials to be updated in fall 2018

Biosecurity Coordinator


The first checklist item recommends designating a Biosecurity Coordinator, who “…is responsible for the development, implementation, maintenance and ongoing effectiveness of the biosecurity program.” The Information Manual for Implementing Poultry Biosecurity (Updated July 2018!) provides guidance for a Biosecurity Coordinator in accomplishing the other checklist items.

pdf_icon Information Manual for Implementing Poultry Biosecurity | pdf_icon Biosecurity Officer Manual – Spanish


Training Employees and Other Personnel**

Short video presentations are available for training purposes. The PowerPoint file for each presentation is also provided. The presentations can be downloaded and modified to better address specific factors at your production site.

**Video presentations to be updated in fall 2018

Do NOT Bring Avian Influenza to the Site

video Video (7 min.)

video Spanish Video (6 min.)
 Spanish Presentation


The resources below are handouts and signs for training
employees, service crews and visitors.***

***Handouts and signs to be updated in fall 2018

Line of Separation Handouts

Line of Separation
DOCX  |   PDF |  Spanish DOCX  |   Spanish PDF

Biosecure Entry Procedure
 DOCX  |   PDF |  Spanish DOCX  |   Spanish PDF

Boot Baths – Footwear Disinfection
 DOCX  |   PDF |  Spanish DOCX  |   Spanish PDF

Perimeter Buffer Area

Perimeter Buffer Area
 DOCX  |   PDF |  DOCX – Spanish  |   PDF – Spanish

Wear Protective Boots
Wear Protective Boots – Spanish


Do NOT Bring Disease to the Site
 DOCX  |   PDF

 DOCX – Spanish  |   PDF – Spanish

BIOS_0440_170925_vector_insectWild Birds, Rodents and Insects

Keep Pests Out 
 DOCX  |   PDF

 DOCX – Spanish  |   PDF – Spanish

Dead Bird Disposal

 DOCX  |   PDF
 DOCX – Spanish  |   PDF – Spanish

BIOS_0440_170925_carcass_manure_disposalManure and Litter Management

 DOCX  |   PDF |  DOCX – Spanish  |   PDF – Spanish